California State University San Marcos
We are always looking for bright motivated trainees to join our research group - please contact me using form at the bottom of this page. Members of groups underrepresented in STEM fields are especially encouraged to apply!
Paid NSF-funded undergraduate summer research opportunities are available in the Department of Biological Sciences (application required, description here)
Dr. Kolosov is available for consultant appointments, expert and technology transfer interviews, science communication and invited seminar talks (please reach out using the form below).
About Kolosov lab
I am interested in how animals work on cellular and molecular levels - there is nothing that piques my interest more than "odd" and "weird" animals that stretch their physiology to the limit! A fish that lives in water and does not explode or shrink because of salt and water imbalance; a caterpillar that grows ~1,000-fold in weeks and eats ~5 times more than it weighs daily; a mosquito that takes a blood meal equivalent to you drinking your own weight in beer and does not explode. All of these animals use specialized epithelia to re-balance their salt and water content and compensate for their crazy lifestyles.
I am a passionate early-career first-generation animal physiologist and educator in the Department of Biological Sciences at CSUSM. My research group at CSUSM will use vertebrate and invertebrate model organisms (e.g., insects and fish) for the study of epithelial ion transport mechanisms. Study of epithelial ion transport in the Kolosov lab will eventually involve the use a variety of cutting-edge laboratory techniques:
- cell and tissue culture, electrophysiology (e.g., membrane potential measurements, ion-selective microelectrodes)
- pharmacology and cell/molecular biology (e.g., RNA extraction, cDNA synthesis, PCR/qPCR, transcriptional knockdown; cloning and heterologous expression)
- bioinformatics (RNAseq, Deseq, GOseq, de novo transcriptome assembly, alternative splicing analysis)
- functional bioassays (e.g., paracellular permeability assays; fluid secretion "Ramsay" assays)
Paid summer research opportunities are available in the Department of Biological Sciences through NSF-funded REU program with several projects taking place in my lab (application required - see details here). Brief description of the projects to be undertaken in the summer of 2021 below.
Transcriptomic survey of animal epithelia for novel mechanisms of rapid regulation of epithelial ion transport
Epithelia are tissues of multicellular organisms that specialize in directional transport of ions and water in and out of the animal. Faced with rapid changes in environmental and systemic variables, animals rely on epithelia to mount a rapid response in a timely manner aimed at maintaining homeostasis. Recent work in my research group has identified several novel molecular mechanisms used by epithelia of the insect ‘kidney’ to rapidly adjust their ion transport. These novel molecular mechanisms include the use of voltage-gated, ligand-gated and mechanosensitive ion channels, as well as the gap junctional coupling between epithelial cells. Despite the fact that these molecular components appear to be very important for the function of insect epithelia, there is no consensus on whether these ion channels are expressed in the epithelia of other animals. Projects use transcriptomics (RNAseq) to (i) identify the above-described ion channels expressed in animal epithelia, and (ii) determine which environmental (e.g., salinity) and systemic (e.g., hormone treatment) factors rely on these ion channels to adjust epithelial function. A mix of lab-generated and publicly available transcriptomic data will be used to carry out these projects.